Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
a-Myb (F5P1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total a-Myb protein. This antibody does not cross-react with b-Myb or c-Myb proteins.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to the transcriptional activation and negative regulatory domains of human a-Myb protein. The antigen has been further categorized as corresponding to residues surrounding Ala383 of human a-Myb protein.
Background
The transcription factor a-Myb, encoded by the gene , is a conserved DNA-binding protein that is critical for both spermatogenesis and mammary gland development (1). Like family members b-Myb and c-Myb, a-Myb contains an N-terminal DNA-binding domain that binds the sequence 5'-YAAC[GT]G-3', a trans-activating domain (TAD), and a C-terminal inhibitory domain (2-4). MYBL1 expression is regulated by cell cycle progression to G1/S phase, and a-Myb protein activity is further regulated by cyclin A/CDK2-dependent phosphorylation of the inhibitory domain (5). a-Myb regulates spermatogenic meiosis through transcriptional control of multiple targets, including RFX2 and Sox30 (6,7). a-Myb and c-Myb N-terminal fusions with the NFI-B transcription factor C-terminus are common oncogenic drivers of salivary gland adenoid cystic carcinoma (ACC) (8-11).
Alternate Names
A-Myb; AMYB; MGC120059; MGC120061; MYB proto-oncogene like 1; Myb-like protein 1; Myb-related protein A; MYBA; MYBL1; v-myb avian myeloblastosis viral oncogene homolog-like 1; v-myb myeloblastosis viral oncogene homolog (avian)-like 1
