Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100 - 1:400
Immunofluorescence (Frozen): 1:400 - 1:1600
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 45083 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
alpha-Synuclein (E4U2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total α-Synuclein protein. This antibody does not cross-react with β-Synuclein. Weak nuclear staining of uncertain specificity was observed in limited ovarian tumors by immunohistochemistry.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human α-Synuclein protein.
Background
α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).
Alternate Names
Alpha-synuclein; alpha-synuclein, isoform NACP140; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); SYUA; truncated alpha synuclein
