Description
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #CUT&RUN Assay Kit # 86652 .
Western Blotting: 1:2000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200 - 1:800
Immunofluorescence (Immunocytochemistry): 1:600 - 1:1200
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:800
Chromatin IP: 1:100
Chromatin IP-seq: 1:100
CUT&RUN: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 23791 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT&RUN
Specificity / Sensitivity
Androgen Receptor (D6F11) Rabbit Monoclonal Antibody detects endogenous levels of total androgen receptor protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.
Background
Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
Alternate Names
AIS; ANDR; Androgen receptor; AR; AR8; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; TFM
