Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
AIFM2/FSP1 (F7A3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total AIFM2/FSP1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AIFM2/FSP1 protein.
Background
Apoptosis-inducing factor mitochondria-associated 2 (AIFM2), also known as AIF-homologous mitochondrion-associated inducer of death (AMID) or p53-responsive gene 3 (PRG3), is a homolog of apoptosis-inducing factor (AIF) (1,2). Research studies show that it inhibits ferroptosis, a form of cell death caused by iron-dependent lipid peroxidation. Therefore, this protein was named as ferroptosis suppressor protein 1 (FSP1). FSP1 is a coenzyme Q (CoQ) oxidoreductase and generates the reduced form of (CoQ), which traps lipid peroxides, leading to the suppression of ferroptosis. Furthermore, inhibition of ferroptosis by FSP1 was shown to be independent of GPX4, another negative regulator of ferroptosis (3,4).
Alternate Names
5430437E11Rik; AIFM2; AMID; apoptosis inducing factor mitochondria associated 2; apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; Apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; apoptosis-inducing factor, mitochondrion-associated, 2; Ferroptosis suppressor protein 1; FSP1; p53-responsive gene 3 protein; PRG3; RP11-367H5.2
