Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ANT1/SLC25A4 (E3E9Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total ANT1/SLC25A4 protein. This antibody does not cross-react with ANT2/SLC25A5 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala150 of human ANT1/SLC25A4 protein.
Background
Adenine nucleotide translocators (ANTs) are a family of mitochondrial proteins that exchange ATP and ADP across the mitochondrial inner membrane. ANT1, also known as solute carrier family 25 member 4 (SLC25A4), is the isoform predominantly expressed in heart, muscle, and brain (1,2). Homozygous loss-of-function mutations in causes cardiomyopathy (2,3). In addition, ANT1/SLC25A4 is shown to be required for mitophagy (3). Furthermore, research studies show that ANT1/SLC25A4 facilitates mitochondrial permeability transition induced by bile acids and, therefore, pyroptosis (4). ANT1/SLC25A4 may mediate the release of ATP from mitochondria to the cytoplasm in this process, which is critical for pyroptosome assembly (4).
Alternate Names
AAC1; Adenine nucleotide translocator 1; adenine nucleotide translocator 1 (skeletal muscle); adenine nucleotide translocator), member 4; ADP,ATP carrier protein 1; ADP,ATP carrier protein, heart/skeletal muscle; ADP,ATP carrier protein, heart/skeletal muscle isoform T1; ADP/ATP translocase 1; ADT1; ANT; ANT 1; ANT1; heart/skeletal muscle ATP/ADP translocator; MTDPS12; MTDPS12A; PEO2; PEO3; PEOA2; SLC25A4; solute carrier family 25 (mitochondrial carrier; Solute carrier family 25 member 4; T1
