Description
Product Usage Information
Reconstitute with 1.5 ml of dH 2 0 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use. Recommended Antibody Dilutions: ELISA (chromogenic substrates): 1:5K-1:100K Western Blotting (chromogenic substrates): 1:5K - 1:100K Western Blotting (ECL substrates): 1:10K - 1:200K Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Storage
Supplied lyophilized with a formulation of 0.01M Sodium Phosphate (pH 7.6), 0.25M NaCl, and 15 mg/ml bovine serum albumin (BSA). Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution, store at -80ºC or add glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.
Specificity / Sensitivity
Anti-Human IgM, Fc5mu Fragment Specific, HRP-Linked Antibody detects the Fc5µ portion of human IgM heavy chain but does not react with human IgG or IgA, or the light chains of human immunoglobulins. This antibody has minimal cross-reaction with bovine serum proteins. This antibody may cross-react with IgM from other species.
Species Reactivity: Human
Source / Purification
Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody is produced by immunizing goats with human IgM. The Anti-Human IgM, Fc5µ Fragment is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
