Description
Product Usage Information
Reconstitution: With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final mIL-10 concentration of greater than 10 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of mIL-10 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mIL-10 should be greater than 10 μg/ml.
Formulation
With carrier: A 0.22 μm filtered solution of 0.33 mg/ml mIL-10 in PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-10. Carrier free: A 0.22 μm filtered solution of 0.33 mg/ml mIL-10 in PBS, pH 7.2.
Storage
Stable at -20°C for 1 year after receipt. Maintain sterility. Do not store frozen.
Source / Purification
Recombinant mouse IL-10 (mIL-10) Ser19 - Ser178 (Accession # NP_034678) was produced in at Cell Signaling Technology.
Background
IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent infections (6).
