Description
Product Usage Information
This product is the carrier free version of product #3676. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3676
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Acetyl-CoA Carboxylase (C83B10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein.
Species Reactivity: Human, Mouse, Rat, Hamster
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1.
Background
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
Alternate Names
ACAC; ACACA; ACACAD; ACACB; ACC; ACC-alpha; ACC-beta; ACC1; ACC2; ACCA; ACCB; Acetyl-CoA carboxylase 1; Acetyl-CoA carboxylase 2; acetyl-CoA carboxylase alpha; acetyl-CoA carboxylase beta; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha; acetyl-Coenzyme A carboxylase beta; Biotin carboxylase; HACC275
