CST, 53331S, Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free)

Product Usage Information
Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) contains 10,000 units and is sensitive to denaturation. Mix by gently inverting the tube. Do not vortex. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.
Storage
Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is a lyophilized powder that contains glycine as a stabilizer. Store at 4ºC and protect from moisture. This product is stable for 12 months when stored at 4ºC. Once in solution, store at -20ºC. Aliquot to avoid multiple freeze/thaw cycles.
Specificity / Sensitivity
One Kunitz unit digests 1 mg of calf thymus DNA in 10 minutes at 37ºC in 50 mM Tris, 1 mM Mg
Source / Purification
Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is chromatographically purified from recombinant bovine pancreatic Deoxyribonuclease I produced in Production in yeast decreases levels of contaminating RNase and eliminates potential pathogens associated with animal-based materials.
Background