CST, 53943S, Human FGF-4 Recombinant Protein

Storage
Human FGF-4 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant human FGF-4 was expressed in and is supplied in a lyophilized form.
Background
FGF-4 is a member of the large and diverse fibroblast growth factor (FGF) family of peptide growth factors. FGF-4 functions as a signaling molecule that is involved in many important processes (1,2). FGF-4 binds to FGFR1 and is required for cardiac gene expression, particularly during second heart field (SHF) proliferation and chamber formation in cooperation with the Sonic hedgehog gene ( ) during heart development (3,4). FGF-4 knockout mice showed developmental defects in embryos thus suggesting that FGF-4 facilitates survival and growth of the inner cell mass during the postimplantation phase of development (1). Since FGFs are involved in gastrointestinal development, upregulation of FGF-4 in pluripotent stem cells has been used to direct their differentiation for the generation of intestinal organoids (5).