Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
2B4/SLAMF4/CD244 (D5J9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total 2B4/SLAMF4/CD244 protein. This antibody cross-reacts with an unidentified protein of 18 kDa in some cell extracts.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp367 of human 2B4/SLAMF4/CD244 protein.
Background
2B4, also known as signaling lymphocytic activation molecule family member 4 (SLAMF4) and cluster of differentiation 244 (CD244), is a heterophilic cell surface receptor expressed on a variety of immune cells, including natural killer (NK) cells, T cells, eosinophils, mast cells, and dendritic cells. 2B4 has been shown to have both immune stimulatory and inhibitory effects on cells (1-11). Upon engagement of its ligand CD48, 2B4 can enhance immune cell signaling, cytokine production, non-major histocompatibility complex-restricted cytotoxicity, and cell migration (1-8). Conversely, at early stages of NK cell differentiation, 2B4 may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (10). 2B4 also inhibits inflammatory responses in dendritic cells (11). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alternate Names
2B4; CD244; CD244 molecule; CD244 molecule, natural killer cell receptor 2B4; h2B4; NAIL; Natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NKR2B4; Nmrk; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4
