Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
ALDH1A2 (E6O6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALDH1A2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro9 of human ALDH1A2 protein.
Background
The aldehyde dehydrogenase family is a large group of enzymes that catalyze the oxidization of aldehydes into carboxylic acids (1). Aldehyde Dehydrogenase 1A2 (ALDH1A2, RALHD2) is among a group of aldehyde dehydrogenases that catalyze the metabolism of retinaldehyde into retinoic acid (RA), which plays a critically important signaling role in animal development (2). Research studies have shown that ALDH1A2 also plays a role postnatally in modulating the effects of RA signaling on immune cell function (3-5). In one example using a genetic mouse model, it was shown that ALDH1A2-dependent RA signaling was a downstream mediator of NOTCH-dependent T cell differentiation (6).
Alternate Names
AL1A2; aldehyde dehydrogenase 1 family member A2; aldehyde dehydrogenase 1 family, member A2; Aldehyde dehydrogenase family 1 member A2; ALDH1A2; MGC26444; RALDH 2; RALDH(II); RALDH2; RALDH2-T; Retinal dehydrogenase 2; Retinaldehyde-specific dehydrogenase type 2
