Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
AP2A2 (E9Q1O) Rabbit Monoclonal Antibody recognizes endogenous levels of total AP2A2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro800 of human AP2A2 protein.
Background
The adaptor-protein 2 (AP-2) coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ, and Ï protein subunits. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into clathrin-coated vesicles destined for incorporation into the endosomal compartment. AP-2 complex subunit alpha-2 (AP2A2) is one of two large alpha-type adaptins that exist within the AP-2 complex. Research studies have shown that AP2A2 works in concert with AP-2 sigma subunits to facilitate the recognition of [ED]-X-X-X-L-[LI] endocytic motifs embedded within the cytoplasmic tails of membrane cargo proteins destined for the endosomal compartment (4).
Alternate Names
100 kDa coated vesicle protein C; Adapter-related protein complex 2 alpha-2 subunit; adapter-related protein complex 2 subunit alpha-2; adaptin, alpha B; Adaptor protein complex AP-2 subunit alpha-2; adaptor related protein complex 2 alpha 2 subunit; adaptor related protein complex 2 subunit alpha 2; Adaptor-related protein complex 2 subunit alpha-2; adaptor-related protein complex 2, alpha 2 subunit; ADTAB; Alpha-adaptin C; Alpha2-adaptin; AP-2 complex subunit alpha-2; AP2A2; CLAPA2; Clathrin assembly protein complex 2 alpha-C large chain; clathrin-associated/assembly/adaptor protein, large, alpha 2; HIP-9; HIP9; Huntingtin interacting protein J; Huntingtin yeast partner J; Huntingtin-interacting protein 9; Huntingtin-interacting protein J; HYPJ; KIAA0899; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
