Description
Product Usage Information
This product is the carrier free version of product #3796. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3796
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
alpha-Amylase (D55H10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total α-amylase protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys288 of human α-amylase.
Background
α-amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). Studies reported the release of an ectopically expressed α-amylase in certain tumors (1). Furthermore, a new type of α-amylase (carcinoid α-amylase or AMY2B) was identified in a lung carcinoid tissue (2-4). The ectopic expression of α-amylase in a neuroendocrine tumor was also reported (5).
Alternate Names
1,4-alpha-D-glucan glucanohydrolase 2B; Alpha-amylase 2B; alpha-amylase carcinoid; AMY2; AMY2B; AMY3; amylase alpha 2B; amylase alpha 2B (pancreatic); amylase, alpha 2B (pancreatic); Carcinoid alpha-amylase; glycogenase; HXA
