Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AGK (E1C6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total AGK protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val192 of human AGK protein.
Background
Acylglycerol kinase (AGK) is a mitochondrial lipid kinase that phosphorylates monoacylglycerol and diacylglycerol to generate bioactive phospholipids lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (1,2). Research studies show that the kinase activity of AGK is required for the activation of PI3K-mTOR signaling and the subsequent enhancement of glycolysis in activated CD8 T cells. Therefore, AGK is essential for the growth, proliferation, and antitumor function of activated CD8 T cells (3). AGK is also a subunit of the mitochondrial TIM22 protein import complex independent of its kinase activity. AGK is critical for the stabilization of the TIM22 complex (2,4).
Alternate Names
acylglycerol kinase; Acylglycerol kinase, mitochondrial; AGK; CATC5; CTRCT38; FLJ10842; hAGK; HsMuLK; MTDPS10; MULK; Multi-substrate lipid kinase; Multiple substrate lipid kinase
