Description
Product Usage Information
Recommended Antibody Dilutions: Western Blotting - 1:1000-1:3000 ELISA-peptide - 1:1000
Storage
Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% Glycerol and less than 0.02% sodium azide. Store at -20C. Do not aliquot antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Anti-biotin (D5A7) Rabbit Monoclonal Antibody recognizes biotin attached to proteins, peptides, oligonucleotides, or solid matrices.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a biotinylated protein.
Background
Biotin is a water-soluble B complex vitamin (vitamin H or B7) that serves as a coenzyme in various metabolic functions. It transfers carbon dioxide for five carboxylase enzymes and is involved in the TCA cycle and gluconeogenesis. In addition, all cells require biotin for cell proliferation, production of fatty acids, and metabolism of fats and amino acids (1). Biotin recycling and attachment to histone proteins is catalyzed by an enzyme named biotinidase. Histone biotinylation is involved in regulating telomere attrition and cellular senescence. Biotinidase deficiency is a rare genetic disorder that can cause neurological and cutaneous symptoms if left untreated (2). Biotin has a high affinity for its natural ligand, avidin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the avidin-biotin complexes (3). This remarkable affinity has been exploited for numerous immunoassays, including ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, Affinity Chromatography, and Immunohistochemistry. Anti-biotin (D5A7) Rabbit mAb #5597 can be used as an alternative to avidins in order to minimize background and maximize signal intensity.
