Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
APOBEC3A (E6K2O) Rabbit Monoclonal Antibody recognizes endogenous levels of total APOBEC3A protein. This antibody does not cross-react with APOBEC3B or APOBEC3G proteins. Based on the sequence of the immunogenic peptide, this antibody is not expected to detect isoform 2 of APOBEC3A.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro9 of human APOBEC3A protein.
Background
Members of the APOBEC3 subfamily of cytosine deaminases (APOBEC3A, APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F, APOBEC3G, and APOBEC3H) function in innate immunity by preventing viral DNA replication, including that of HIV-1 (1-2). APOBEC3A expression is upregulated in several human cancers, and is a major source of mutation in breast cancer (3). APOBEC3A contributes to chromosomal instability and disease progression in pancreatic cancer (4), and upregulates PD-L1 expression in cancer cells (5).
Alternate Names
A3A; ABC3A; APOBEC3A; apolipoprotein B mRNA editing enzyme catalytic subunit 3A; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A; ARP3; bK150C2.1; DNA dC->dU-editing enzyme APOBEC-3A; phorbolin 1; Phorbolin-1; PHRBN; Probable DNA dC->dU-editing enzyme APOBEC-3A
