CST, 58948S, Human Fc Receptor Blocking Solution

Product Usage Information
Prior to immunostaining, treat cells with Human Fc Receptor Blocking Solution for 10 min at room temperature, using 2.5 μg/1 million cells in approximately 100 μL Antibody Dilution Buffer (may be purchased as ready-to-use Flow Cytometry Antibody Dilution Buffer # 13616 or prepared as a solution of 0.5% Bovine Serum Albumin in 1X Phosphate Buffered Saline). No wash step is needed before incubating cells with primary antibodies.
Storage
Supplied in PBS solution (pH 7.2). Stable at 4°C for 1 year after receipt. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Specificity / Sensitivity
Species Reactivity: Human
Source / Purification
Modified human IgG1 was expressed in 293 cells.
Background
Human Fc Receptor Blocking Solution contains a modified form of human IgG1 that blocks human Fc receptors.