Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AQP5 (F6X2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total AQP5 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other aquaporin family members. By western blot, this antibody may detect a band of unknown origin at approximately 12 kDa in some samples.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu262 of human AQP5 protein.
Background
Aquaporin 5 (AQP5) is a plasma membrane water channel whose overexpression in human cancers, such as breast, pancreatic, and colon cancers, has been associated with poor prognosis and metastasis. In colorectal cancer cells, AQP5 has been shown to regulate migration and invasion, as well as expression of epithelial-mesenchymal transition (EMT) factors. Knockdown of AQP5 inhibits the Wnt/β-Catenin pathway (1). Localization of AQP5 is altered in gastric cancer, and AQP5 is a marker for pyloric stem cells (2). In hepatocellular carcinoma (HCC), downregulation of AQP5 inhibits metastasis and EMT through inactivation of the NF-κB signaling pathway, and therefore, AQP5 is a potential therapeutic target for HCC (3). Phosphorylation of AQP5 at Ser156, possibly by PKA, activates the Ras signaling pathway in NIH/3T3 cells (4). AQP5 alters cell shape and actin dynamics without inducing EMT in normal epithelial Madin-Darby canine kidney (MDCK) cells, and this effect likely requires phosphorylation of AQP5 at Ser156 (5).
Alternate Names
AQP-5; AQP5; aquaporin 5; Aquaporin-5; PPKB
