Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
A2BP1 (E8R9Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total A2BP1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro106 of human A2BP1 protein.
Background
Ataxin-2-binding protein 1 (A2BP1) is part of the RNA-binding Fox (RBFOX) family of splicing factors, which is composed of three members, RBFOX1 (Fox-1 or A2BP1), RBFOX2 (Fox-2 or RBM9), and RBFOX3 (Fox-3, HRNBP3, or NeuN). RBFOX proteins regulate splicing of many neuronal transcripts by binding the sequence (U)GCAUG in mRNA precursors (1). Human A2BP1 was first identified through its interaction with Ataxin-2, the protein mutated in spinocerebellar ataxia type II. It has since been shown to play a role in the etiology of autism spectrum disorder (ASD) (1,2). Impaired A2BP1 function was found to cause abnormal corticogenesis during brain development. The abnormal process may underlie the basic pathophysiology of ASD and other neurodevelopmental disorders and may contribute to the emergence of the clinical symptoms of patients with gene abnormalities (3). The expression of A2BP1 is restricted to neurons, heart, and muscle (1,4).
Alternate Names
2BP1; A2BP; A2BP1; ataxin 2-binding protein 1; Ataxin-2-binding protein 1; FOX-1; Fox-1 homolog A; fox-1-like RNA-binding protein 1; FOX1; FOX1A; hexaribonucleotide binding protein 1; Hexaribonucleotide-binding protein 1; HRNBP1; RBFOX1; RFOX1; RNA binding fox-1 homolog 1; RNA binding protein fox-1 homolog 1; RNA binding protein, fox-1 homolog 1
