Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75018 .
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ApoE2 (E6U9I) Rabbit Monoclonal Antibody recognizes overexpressed levels of total ApoE2 protein. This antibody does not cross-react with ApoE3 or ApoE4 by western blot. This antibody may detect non-specific bands of unknown origin at 55 kDa and 70 kDa.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys176 of human ApoE2 protein.
Background
Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2). Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE2 isoform contains cysteine at both locations. ApoE4 has been identified as a genetic risk factor for Alzheimer's disease (AD), where ApoE2 is neuroprotective, reducing risk of AD by nearly 50%. The neuroprotective mechanism of ApoE2 remains under investigation, but studies suggest that this may be due to its overall protein abundance in non-AD human cortex and its unique lipid binding profile compared to ApoE3 and ApoE4, which may increase overall β-amyloid clearance (3).
Alternate Names
AD2; Apo-E; APOE; Apolipoprotein E; LDLCQ5; LPG; MGC1571
