Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ACF1 Antibody recognizes endogenous levels of total ACF1 protein (isoforms 1 and 2).
Species Reactivity: Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met864 of human ACF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The mammalian imitation SWI (ISWI) complexes are characterized by two ATPase subunits: Snf2h and Snf2l (1). Snf2h interacts with ATP-utilizing chromatin assembly and remodeling factor 1 (ACF1) to comprise the ACF chromatin-remodeling complex (1). ACF1 (BAZ1A) has distinct roles in development (2), regulation of chromatin structure (3), and DNA damage response (4,5). Different developmental stages dictate the expression of ACF1 in Drosophila, and alterations in ACF1 expression during Drosophila development leads to deviation from normal chromatin organization (2). ACF1 functions in heterochromatin formation during development and is involved in the initial establishment of diversified chromatin structures. studies demonstrate that heterochromatin protein 1 (HP1) binding to methylated lysine 9 of histone H3 is enhanced by the interaction of ACF1 with chromatin (6). Chromatin-remodeling factors are required during DNA damage in order to allow signaling molecules and damaging enzymes to access the site (4). Depletion of hACF1 increases apoptosis and vulnerability to radiation and compromises G2/M arrest activated in response to X-ray and UV exposure (4). Depletion of ACF1 also sensitizes cells to DNA double-stranded breaks (DSBs) and impairs DNA repair (5). Specifically, accumulation of Ku at DSBs sites may depend on the presence of ACF1 (5).
Alternate Names
ACF1; ATP-dependent chromatin remodeling protein; ATP-dependent chromatin-remodeling protein; ATP-utilizing chromatin assembly and remodeling factor 1; BAZ1A; bromodomain adjacent to zinc finger domain 1A; Bromodomain adjacent to zinc finger domain protein 1A; bromodomain adjacent to zinc finger domain, 1A; CHRAC subunit ACF1; DKFZp586E0518; FLJ14383; hACF1; hWALp1; WALp1; WCRF180; Williams syndrome transcription factor-related chromatin-remodeling factor 180
