Description
Product Usage Information
This product is the carrier free version of product #94019. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 94019
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
15-PGDH/HPGD (E9H7W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total 15-PGDH/HPGD protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human 15-PGDH/HPGD protein.
Background
15-hydroxyprostaglandin dehydrogenase (15-PGDH or HPGD) catalyzes the rate-limiting step of prostaglandin E2 (PGE2) degradation (1). Its inhibition was shown to potentiate tissue regeneration in various mouse organs (2). Increased 15-PGDH/HPGD levels are a hallmark of aged skeletal muscles. 15-PGDH/HPGD inhibition in aged mouse muscles leads to elevated PGE2 levels and boosts mitochondrial biogenesis and function, enhancing muscle mass and strength (3). Studies show that loss of 15-PGDH/HPGD also leads to increased hematopoietic output and enhanced gastrointestinal fitness in aged mice, indicating a critical role of this enzyme in the regulation of tissue regeneration during aging (1). In addition, PPARγ upregulates15-PGDH/HPGD expression in the regulatory T cells in visceral adipose tissue, suppressing conventional T cell activation and proliferation (4).
Alternate Names
15-hydroxyprostaglandin dehydrogenase; 15-hydroxyprostaglandin dehydrogenase (NAD(+)); 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; 15-hydroxyprostaglandin dehydrogenase [NAD+]; 15-PGDH; HPGD; hydroxyprostaglandin dehydrogenase 15-(NAD); NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; PGDH; PGDH1; PHOAR1; Prostaglandin dehydrogenase 1; SDR36C1; Short chain dehydrogenase/reductase family 36C member 1; short chain dehydrogenase/reductase family 36C, member 1
