CST, 63228S, CUT&Tag PCR Master Mix

Storage
Store at -20ºC. This product is stable for 18 months if stored properly.
Protocol
Available protocols: CUT&Tag
Specificity / Sensitivity
Species Reactivity: All Species Expected
Background
Similar to Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag) is a powerful technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-3). CUT&Tag has many of the same advantages as the CUT&RUN assay in that it provides a rapid, robust, and true low cell number protocol for detection of protein-DNA interactions in the cell. In addition, the CUT&Tag assay adds an adaptor DNA ligation step carried out by the pAG-Tn5 enzyme, in which an adaptor DNA is ligated directly to antibody-targeted chromatin DNA fragments in the cell. As a result, subsequent DNA library preparation is much faster and easier than library preparation following the CUT&RUN assay, free from DNA end repair, A-tailing, and adaptor ligation . CUT&Tag works very well for analyzing histone modifications, in addition to mapping some transcription factor and cofactor binding.
Alternate Names
amplification; C&T; cut and tag; cut&tag; CUT&Tag; cutandtag; library; PCR; polymerase