Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ARALAR/AGC1 (D5I6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARALAR/AGC1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg309 of human ARALAR/AGC1 protein.
Background
Mitochondrial carriers are integral proteins of the mitochondrial inner membrane that transport metabolites, nucleotides, and co-factors between the cytosol and the mitochondria (1). The calcium-binding mitochondrial carrier protein ARALAR (SLC25A12, AGC1) is an aspartate-glutamate exchange protein responsible for transporting mitochondrial aspartate across the mitochondrial inner membrane in exchange for cytosolic glutamate (2,3). ARALAR and other proteins of the aspartate-glutamate carrier (AGC) group are required for the transfer of mitochondrial aspartate to the cytosol, a key step in urea synthesis (4). Research studies using ARALAR-knockout mice indicate that ARALAR plays an important role in proper CNS myelination. Mice lacking ARALAR suffer from hypomyelination as a result of a lack of oligodendrocyte maturation caused by decreased brain N-acetylaspartate levels (5). Mutation of the corresponding SLC25A12 gene can result in global cerebral hypomyelination and severe psychomotor retardation, caused by deficient ARALAR activity and limited mitochondrial aspartate efflux (6).
Alternate Names
AGC1; araceli hiperlarga; ARALAR; ARALAR1; calcium binding mitochondrial carrier superfamily member Aralar1; Calcium-binding mitochondrial carrier protein Aralar1; CMC1; EIEE39; Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial; Mitochondrial aspartate glutamate carrier 1; SLC25A12; solute carrier family 25 (aspartate/glutamate carrier), member 12; solute carrier family 25 (mitochondrial carrier, Aralar), member 12; Solute carrier family 25 member 12
