Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ACSL5 (F8M1Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACSL5 protein. This antibody does not cross-react with ACSL1, ACSL3, or ACSL4 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ACSL5 protein.
Background
Acyl-CoA synthetase long-chain family member 5 (ACSL5), also known as a long-chain acyl-CoA synthetase 5 (LACS 5), catalyzes the conversion of long-chain fatty acids to corresponding acyl-CoAs in a two-step reaction, activating long-chain fatty acids (1,2,4). Studies show that SIRT6 binds palmitic acid, leading to its translocation from the nucleus to the cytoplasm. SIRT6 then deacetylates ACSL5, stimulating fatty acid oxidation and inhibiting lipid accumulation. Furthermore, these findings suggest a role of cytoplasmic SIRT6/ACSL5 signaling in nonalcoholic fatty liver disease (NAFLD) progression (3). Additionally, ACSL5 has been shown to function as an immune-dependent tumor suppressor. ACSL5 sensitizes tumor cells to CD8 T cell-mediated cytotoxicity and PD-1 blockade therapy .
Alternate Names
ACS2; ACS5; ACSL5; acyl-CoA synthetase long chain family member 5; acyl-CoA synthetase long-chain family member 5; Arachidonate--CoA ligase; FACL5; FACL5 for fatty acid coenzyme A ligase 5; fatty acid coenzyme A ligase 5; fatty-acid-Coenzyme A ligase, long-chain 5; LACS 5; Long-chain acyl-CoA synthetase 5; long-chain fatty acid coenzyme A ligase 5; Long-chain-fatty-acid--CoA ligase 5
