CST, 6813S, BrdU Cell Proliferation Assay Kit

Protocol
Available protocols: Functional
Specificity / Sensitivity
BrdU Cell Proliferation Assay Kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU (Bu20a) Mouse Monoclonal Antibody used in this kit. This antibody does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x10
Species Reactivity: All Species Expected
Background
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4).