Description
Product Usage Information
Western Blotting: 1:1000
Fluorescent Western: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
alpha-Actinin (E7U1O) Mouse Monoclonal Antibody recognizes endogenous levels of total α-Actinin protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-Actinin-1 protein.
Background
α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. The α-actinin protein interacts with a large number of proteins involved in signaling to the cytoskeleton, including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). The cytoskeletal α-actinin isoforms 1 and 4 (ACTN1, ACTN4) are non-muscle proteins that are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 (ACTN2, ACTN3) localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).
Alternate Names
actinin 1 smooth muscle; actinin alpha 1; actinin, alpha 1; ACTN1; alpha-actinin; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; epididymis secretory sperm binding protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1
