Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ANKRD15 (E9I4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total ANKRD15 protein. This antibody detects a nonspecific band at 48 kDa by western blot.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ANKRD15 protein.
Background
ANKRD15 (ankyrin repeat domain-containing protein 15, KANK1) belongs to the Kank family of proteins, which contain multiple ankyrin repeat domains and are essential for crosstalk between actin and microtubules (1). ANKRD15 was initially identified as a potential tumor suppressor for renal cell carcinoma and has since been linked to gastric cancer, colorectal cancer, and oral squamous carcinoma (1,2). The protein regulates actin polymerization and cell motility and has two major alternatively spliced transcripts with different promoters and several other possible transcripts (3). Loss-of-function mutations have since been linked to congenital cerebral palsy and other neurodevelopmental disorders (3,4).Â
Alternate Names
ANKRD15; ankyrin repeat domain 15; Ankyrin repeat domain-containing protein 15; CPSQ2; DKFZp451G231; KANK; KANK1; KIAA0172; Kidney ankyrin repeat-containing protein; KN motif and ankyrin repeat domain-containing protein 1; KN motif and ankyrin repeat domains 1; MGC43128
