Description
Product Usage Information
For more information about the RBP-eCLIP service please visit Eclipsebio .
Western Blotting: 1:1000
Immunoprecipitation: 1:50
eCLIP: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
alpha-Adducin (D7T7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total α-adducin protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp643 of human α-adducin protein.
Background
The adducins (ADD) are cytoskeleton-associated proteins that help cap the ends of actin filaments, promote association between spectrin and actin, and participate in synapse assembly. The three closely related genes , , and encode the α-adducin, β-adducin, and γ-adducin proteins (1). Research studies indicate that β-adducin is found at high levels in brain and hematopoietic tissues, whereas both α-adducin and γ-adducin are ubiquitously expressed (2). Adducin protein function is regulated by phosphorylation at a number of sites. Both PKA and PKC can phosphorylate α-adducin at Ser726 and β-adducin at Ser713, which inhibits calmodulin binding and adducin activity (3-5). Additionally, PKA (but not PKC) can phosphorylate β-adducin at Ser408, Ser436, and Ser481, which negatively affects spectrin-actin interactions (3). Phosphorylation of α-adducin at Thr445 and Thr480 by Rho-kinase regulates cell motility and membrane ruffling (6). Finally, CDK-1 phosphorylation of α-adducin at Ser12 and Ser355 during mitosis leads to association of α-adducin with the mitotic spindle, suggesting that α-adducin may play a role in mitotic regulation (7). Because α-adducin plays a role in regulating renal sodium reabsorption, it is not surprising that a number of studies show a relationship between genetic polymorphisms and the development of hypertension (8-10).
Alternate Names
ADD1; ADDA; adducin 1; adducin 1 (alpha); Alpha-adducin; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427
