CST, 7074S, Anti-rabbit IgG, HRP-linked Antibody

Product Usage Information
Recommended Antibody Dilutions: 1:1000-1:3000 20X LumiGLO ® Reagent and 20X Peroxide #7003 1:1K-1:3K SignalFire™ ECL Reagent #6883 1:1K-1:3K SignalFire™ Plus ECL Reagent #12630 1:5K-1:15K SignalFire™ Elite ECL Reagent #12757 1:10K-1:20K
Storage
Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at -20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Species Reactivity: Rabbit
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.