Description
Product Usage Information
Western Blotting: 1:2000
IHC Leica Bond: 1:400 - 1:1600
Immunohistochemistry (Paraffin): 1:200 - 1:800
Storage
Supplied in PBS (pH 7.3), 1% BSA, 0.02% sodium azide, and 50% glycerol. Stable for 12 months when stored at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
ACE2 (OTI1G4) Mouse Monoclonal Antibody recognizes endogenous levels of ACE2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to amino acids 18-237 of human ACE2 protein.
Background
ACE2 is a carboxypeptidase that catalyses the conversion of angiotensin I to angiotensin 1-9, or of angiotensin II to the vasodilator angiotensin 1-7 (1). ACE2 is a critical component in the renin-angiotensin system (RAS). ACE2 is predominantly expressed in vascular endothelial cells of the heart and kidney and Leydig and Sertoli cells of the testis (2,3). The unique expression pattern of ACE2 determines its essential role in the regulation of cardiovascular and kidney functions, as well as fertility. ACE2 protein is localized mainly in the extracellular space with its carboxy-terminal end attached to the membrane via its transmembrane domain. Active ACE2 enzyme is secreted by cleavage at the amino terminus. Research studies have shown that ACE2 expression is elevated in human failing heart (4). ACE2 has also been identified as the receptor for SARS and SARS-CoV-2 coronaviruses (5-7).
Alternate Names
ACE-related carboxypeptidase; ACE2; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; DKFZp434A014; Metalloprotease MPROT15; peptidyl-dipeptidase A; Processed angiotensin-converting enzyme 2; truncated angiotensin converting enzyme 2
