Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
APEX2 (E5A2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total APEX2 protein. This antibody cross-reacts with an aproximately 100 kDa band of unknown origin by western blot.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu393 of human APEX2 protein.
Background
DNA-(apurinic or apyrimidinic site) endonuclease 2 (APEX2/APE2) is an endodeoxyribonuclease that functions in the DNA base excision repair (BER) pathway to repair DNA lesions induced by oxidative and alkylating agents. APEX2 acts in ATR-dependent DNA damage checkpoint activation in response to oxidative stress (1). The gene is synthetically lethal with and genes, which are required for homologous recombination (HR) repair of double-strand DNA breaks. This is due to the ability of APEX2 to reverse single-strand DNA breaks with 3' end blocks. Because BRCA-deficient cancer cells are sensitive to 3' blocks, APEX2 is a potential therapeutic target for HR-deficient cancers (2,3).
Alternate Names
AP endonuclease 2; AP endonuclease XTH2; APE2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; APEX nuclease 2; APEX nuclease-like 2; APEX2; APEXL2; Apurinic-apyrimidinic endonuclease 2; apurinic/apyrimidinic endodeoxyribonuclease 2; apurinic/apyrimidinic endonuclease-like 2; DNA-(apurinic or apyrimidinic site) endonuclease 2; DNA-(apurinic or apyrimidinic site) lyase 2; XTH2; ZGRF2; zinc finger, GRF-type containing 2
