Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ALAD (E8Z7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALAD protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human ALAD protein.
Background
Delta-aminolevulinic acid dehydratase (ALAD) catalyzes the second step in heme biosynthesis. ALAD is also an endogenous inhibitor of the 26S proteasome (1). Defects in ALAD cause acute hepatic porphyria, an inherited defect in heme biosynthesis in the liver (2). ALAD activity is inhibited by lead, and defects in ALAD result in sensitivity to lead poisoning (3). Reduced expression of ALAD is associated with poor prognosis in breast cancer patients. Overexpression of ALAD in breast cancer cells negatively regulates proliferation, invasion, and transforming growth factor-β (TGF-β)-mediated epithelial-mesenchymal transition (EMT) (4).
Alternate Names
ALAD; ALADH; aminolevulinate dehydratase; aminolevulinate, delta-, dehydratase; Delta-aminolevulinic acid dehydratase; HEM2; MGC5057; PBGS; Porphobilinogen synthase; testicular tissue protein Li 95
