CST, 78450S, Ammonium Bicarbonate, 1M in HPLC-Grade Water

Product Usage Information
1M Ammonium bicarbonate buffer in HPLC water is provided for use with Cell Signaling Technology's patented PTMScan ® protocol. Secondary trypsin digestion of enriched LysC or Methylated peptides is recommended for all basophilic and methylation-specific motif antibodies. Use this 1M ammonium bicarbonate (20X) stock to prepare the final digestion buffer of 50mM ammonium bicarbonate with 5% acetonitrile. Consult the specific PTMScan ® kit and protocol for more details on the appropriate protease before digesting any protein samples.
Storage
Aliquoted buffer can be stored at -20°C for long-term storage for 12 months.