Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
4EHP (E4D5S) Rabbit Monoclonal Antibody recognizes endogenous levels of total 4EHP protein. This antibody does not cross-react with eIF4E protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human 4EHP protein.
Background
Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. A mammalian cap-binding protein related to eIF4E, 4E Homologous Protein (4EHP), was later identified (6). 4EHP does not interact with eIF4G and, therefore, cannot activate translation (7). Instead, it inhibits the translation of a subset of mRNAs (7).
Alternate Names
4E-LP; 4EHP; eIF-4E type 2; eIF4E type 2; eIF4E-like cap-binding protein; eIF4E-like protein 4E-LP; EIF4E2; EIF4EL3; eukaryotic translation initiation factor 4E family member 2; Eukaryotic translation initiation factor 4E homologous protein; eukaryotic translation initiation factor 4E member 2; Eukaryotic translation initiation factor 4E type 2; Eukaryotic translation initiation factor 4E-like 3; h4EHP; IF4e; IF4E2; mRNA cap-binding protein 4EHP; mRNA cap-binding protein type 3
