Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Aldolase A (D73H4) Rabbit Monoclonal Antibody recognizes endogenous levels of total fructose bisphosphate aldolase A protein. This antibody may detect total fructose bisphosphate aldolase B and fructose bisphosphate aldolase C proteins.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human fructose bisphosphate aldolase A protein.
Background
Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma (1). Furthermore, investigators have shown that changes in aldolase B gene expression levels have been observed in certain patients with this primary tumor (2,3).
Alternate Names
ALDA; ALDOA; aldolase A, fructose-bisphosphate; aldolase, fructose-bisphosphate A; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase A; GSD12; HEL-S-87p; Lung cancer antigen NY-LU-1; MGC10942; MGC17716; MGC17767; Muscle-type aldolase
