CST, 81178S, Beta-d-N4-Hydroxycytidine

Product Usage Information
Beta-d-N4-Hydroxycytidine is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.28 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
Beta-d-N4-Hydroxycytidine, also known as EIDD-1931, is a nucleic acid base analog and a specific AT to GC mutagen formed by the treatment of nucleoside cytidine with hydroxylamine (1). Beta-d-N4-Hydroxycytidine acts as a competitive substrate for viral encoded RNA polymerase and has demonstrated broad antiviral activity, including Chikungunya virus, Ebola, and Hepatitis C (2-4). Beta-d-N4-Hydroxycytidine demonstrated an IC value of 0.1 µM and an IC value of 6 µM in SARS-CoV infected Vero cells (5,6).
Alternate Names
1-[2R3R4S5R-34-Dihydroxy-5-hydroxymethyloxolan-2-yl]-4-hydroxyaminopyrimidin- 2-one; D-N4-hydroxycytidine; EIDD-1931; EIDD1931; N4-Hydroxycytidine; NHC