Description
Product Usage Information
Western Blotting: 1:1000
Flow Cytometry (Fixed/Permeabilized): 1:500 - 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
ALOX15 (E5D1C) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALOX15 protein. This antibody does not cross-react with ALOX15B protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a region of human ALOX15 protein.
Background
Polyunsaturated fatty acid lipoxygenase ALOX15, also known as arachidonate 15-lipoxygenase (15-LOX, 15-LOX-1), catalyzes oxygenation of polyunsaturated fatty acids to corresponding hydroperoxides (1,2). Studies show that the small scaffolding protein PEBP1 associates with ALOX15 and polyunsaturated fatty acid lipoxygenase ALOX15B to change their substrate specificity, resulting in the conversion of polyunsaturated phosphatidylethanolamines (PE) to hydroperoxy-PE. Hydroperoxy-PE, when not sufficiently reduced by GPX4, causes ferroptosis (3). In addition, a loss-of-function mutation in ALOX15 leads to protection against nasal polyps and chronic rhinosinusitis (4). Furthermore, ALOX15-positive macrophages contribute to the pathogenesis of a subtype of chronic rhinosinusitis (5).
Alternate Names
12-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; 15-lipoxygenase type 1; 15-LOX; 15-LOX type 1; 15-LOX-1; 15LOX-1; ALOX15; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; LOG15; LOX15; Polyunsaturated fatty acid lipoxygenase ALOX15
