Description
Product Usage Information
Immunofluorescence (Frozen): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
AQP4 (D1F8E) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human AQP4 protein.
Background
Aquaporins (AQPs) are integral membrane proteins that serve as channels to transfer water and small solutes across the membrane. There are 13 isoforms of AQP that are expressed in different types of cells and tissues (1,2). AQP1 is found in blood vessels, kidney, eye, and ear. AQP2 is found in the kidney, and it has been shown that the lack of AQP2 results in diabetes (1,3). AQP4 is present in the brain, where it is enriched in astrocytes (1,2,4). AQP5 is found in the salivary and lacrimal gland, AQP6 in intracellular vesicles in the kidney, AQP7 in adipocytes, AQP8 in kidney, testis, and liver, AQP9 is present in liver and leukocytes, and AQP10-11 in the intestine (1,3,4). AQPs are essential for the function of cells and organs. It has been shown that AQP1 and AQP4 regulate water homeostasis in astrocytes, preventing cerebral edema caused by solute imbalance (5). Several studies have shown the involvement of AQPs in the development of inflammatory processes, including innate and adaptive cell immunity (6,7).
Alternate Names
AQP-4; AQP4; aquaporin 4; aquaporin type4; Aquaporin-4; HMIWC2; Mercurial-insensitive water channel; MGC22454; MIWC; WCH4
