Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ACSL3 (E2S9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACSL3 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu529 of human ACSL3 protein.
Background
Mammalian long-chain acyl-CoA synthetase (LACS or ACSL) catalyzes the ligation of fatty acids to CoA to form fatty acyl-CoA in a two-step reaction. Five isoforms of ACSL have been identified. These isoforms have different substrate preferences and subcellular localizations (1). Studies show that exogenous monounsaturated fatty acids inhibit ferroptosis and that this process requires the activation of monounsaturated fatty acids by acyl-CoA synthetase long-chain family member 3 (ACSL3) (2). In addition, studies on a mouse model show that Acsl3 is essential for oleic acid to protect melanoma cells from ferroptosis, which leads to more tumor metastases (3). Furthermore, ACSL3 is required for prostaglandin synthesis and tumorigenesis in non-small cell lung cancer (NSCLC) (4).
Alternate Names
ACS3; ACSL3; acyl-CoA synthetase long chain family member 3; acyl-CoA synthetase long-chain family member 3; Arachidonate--CoA ligase; FACL3; Fatty acid CoA ligase Acsl3; fatty-acid-Coenzyme A ligase, long-chain 3; LACS 3; LACS3; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 3; Long-chain-fatty-acid--CoA ligase 3; PRO2194
