Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ALDH1A2 Antibody recognizes endogenous levels of total ALDH1A2 protein. Based on sequence comparisons, this antibody is not predicted to cross-react with ALDH1A1.
Species Reactivity: Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly350 of human ALDH1A2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The aldehyde dehydrogenase family is a large group of enzymes that catalyze the oxidization of aldehydes into carboxylic acids (1). Aldehyde Dehydrogenase 1A2 (ALDH1A2, RALHD2) is among a group of aldehyde dehydrogenases that catalyze the metabolism of retinaldehyde into retinoic acid (RA), which plays a critically important signaling role in animal development (2). Research studies have shown that ALDH1A2 also plays a role postnatally in modulating the effects of RA signaling on immune cell function (3-5). In one example using a genetic mouse model, it was shown that ALDH1A2-dependent RA signaling was a downstream mediator of NOTCH-dependent T cell differentiation (6).
Alternate Names
AL1A2; aldehyde dehydrogenase 1 family member A2; aldehyde dehydrogenase 1 family, member A2; Aldehyde dehydrogenase family 1 member A2; ALDH1A2; MGC26444; RALDH 2; RALDH(II); RALDH2; RALDH2-T; Retinal dehydrogenase 2; Retinaldehyde-specific dehydrogenase type 2
