CST, 84566S, (+)-JQ1

Product Usage Information
(+)-JQ1 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.09 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Background
(+)-JQ1 is a potent BET bromodomain inhibitor with IC values of 77 nM and 33 nM for BRD4 bromodomain 1 and BRD4 bromodomain 2, respectively. This small molecule competitively binds to the acetyl-lysine recognition pocket of BET bromodomains, which in turn displaces BET proteins from chromatin (1). The BET family of proteins are associated in many different cellular processes, including mitosis, viral/host interaction, and inflammatory gene expression (2-4). Studies have shown that (+)-JQ1 induced autophagy through LKB1/AMPK pathway activation can inhibit bladder cancer (BC) cell proliferation (5).
Alternate Names
6S-4-4-Chlorophenyl-239-trimethyl-6H-thieno[32-f][124]triazolo[43-a][14]diazepine-6- acetic acid 11-dimethylethyl ester; JQ-1; JQ1