Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AIRE (F5F8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total AIRE protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly240 of human AIRE protein.
Background
Autoimmune disease is a significant health concern resulting from a breakdown in normal immune self-tolerance. Immune self-tolerance is regulated by a complex system that includes key cell types, regulatory molecules, cytokines, and intricate immune cell selection processes (1-3). The autoimmune regulator (AIRE) is a transcription factor first discovered in autoimmune disease patients with rare genetic mutations (4-6). AIRE has four distinct domains: a caspase recruitment domain (CARD), the SAND domain, and plant homeodomains 1 and 2 (PHD1 and PHD2) (2,3). AIRE plays a vital role in promoting self-tolerance in the thymus by regulating expression of common tissue-restricted peripheral self-antigens called tissue-restricted antigens (TSAs) (3). AIRE is primarily expressed by medullary thymic epithelial cells (mTECs), and coordinates expression of tissue-restricted proteins that are presented on major histocompatibility complex class I and II (MHC-I, MHC-II) and to developing T cells in the thymus (2,3). AIRE facilitates self-tolerance in other ways, such as regulating mTEC differentiation, controlling thymic dendritic cell accumulation, and developing regulatory T cells via XCL1 expression (3). AIRE regulates the production of CCR4 and CCR7 binding cytokines in mTECs and can alter thymocyte development and migration (2,3). In thymic B cells, AIRE is involved in self-antigen presentation for negative selection (2,3).
Alternate Names
AIRE; AIRE1; APECED; APECED protein; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; Autoimmune regulator; PGA1
