CST, 8581S, APR3 Antibody

Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
APR3 Antibody recognizes endogenous levels of total APR3 protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of human APR3 protein (isoform a). Antibodies are purified by protein A and peptide affinity chromatography.
Background
Apoptosis related protein 3 (APR3), also known as C2orf28, is a membrane protein identified in HL-60 cells treated with all-trans retinoic acid (ATRA) and was later found to be induced by ATRA in other sensitive cell lines (1,2). APR3 is also up-regulated by NFAT and NF-κB activities (3). Regulation of APR3 by ATRA suggests a role in cell differentiation, but the mechanism of action is still unclear. Overexpression of APR3 can inhibit proliferation by inducing G1/S cell cycle arrest and decreasing expression of cyclin D1 (2). APR3 has also been shown to interact with NELL-1 to regulate osteoblast differentiation (4).
Alternate Names
all-trans retinoic acid induced differentiation factor; All-trans retinoic acid-induced differentiation factor; apoptosis related protein 3; apoptosis related protein APR-3; Apoptosis-related protein 3; APR--3; APR-3; APR3; ARAID; ATRAID; C2orf28; chromosome 2 open reading frame 28; HSPC013; p18; PRO240