Description
Product Usage Information
Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) # 9803 . See protocol for more details. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack # 7017 . Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.
Immunoprecipitation: 1:20
Storage
Supplied in PBS Buffer (pH 7.2), 0.1% Tween ® 20. Store at 4°C. Do not aliquot the antibodies.
Specificity / Sensitivity
Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (Magnetic Bead Conjugate) is not directed against any known antigen. This antibody conjugate functions as an isotype control for rabbit IgG monoclonal antibodies.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to F receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
