CST, 87548S, Mouse FGF-basic/FGF2 Recombinant Protein

Storage
Mouse FGF-basic/FGF2 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Source / Purification
Recombinant mouse FGF-basic was expressed in and is supplied in a lyophilized form.
Background
FGF-basic (FGF2) is produced in both embryonic and adult cell types, and contributes to the pathogenesis of various diseases, including cancer and atherosclerosis (1). FGF-basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF-basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF-basic has a high affinity for heparan sulfate (1,2) and binding is a step in the FGF-basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF-basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).