Description
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Single Cell Analysis - InTraSeq™ 3'
Specificity / Sensitivity
CD4 (RPA-T4) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3029) recognizes endogenous levels of total CD4 protein. This antibody conjugate detects an epitope within the extracellular domain.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with PHA-stimulated human peripheral blood mononuclear cells.
Background
Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
Alternate Names
CD4; CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
