Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ALKBH3 (E6S4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALKBH3 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val10 of human ALKBH3 protein.
Background
ALKBH3, also known as Prostate Cancer Antigen-1 (PCA-1), is a DNA and RNA demethylase involved in the repair of alkylated DNA. The protein is overexpressed in prostate cancer and has been linked to other cancer types, including non-small cell lung cancer, renal cell carcinoma, and pancreatic carcinoma (1-3). ALKBH3/PCA-1 is also a potential therapeutic target in prostate cancer (4).
Alternate Names
ABH3; AlkB homolog 3; alkB homolog 3, alpha-ketoglutarate dependent dioxygenase; alkB homolog 3, alpha-ketoglutaratedependent dioxygenase; alkB, alkylation repair homolog 3; alkB, alkylation repair homolog 3 (E. coli); ALKB3; ALKBH3; Alkylated DNA repair protein alkB homolog 3; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC-1; DEPC1; FLJ43614; hABH3; MGC118790; MGC118792; MGC118793; PCA1; Prostate cancer antigen 1; prostate cancer antigen-1
