CST, 8783S, LysoTracker ® Green DND-26

Product Usage Information
Each vial of LysoTracker ® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX! To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop ® Green Background Suppressor #12388 can also be added to reduce background fluorescence.
Storage
Store at -20°C dessicated and protected from light. This reagent is stable for 12 months. Seal vial tightly after use to refreeze. Avoid excessive freeze-thaw cycles.
Background
LysoTracker Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.
Alternate Names
acidic compartment; live cell; lyso; Lysosome; Lysosomes; trace; tracker